Larvicidal activity of Illicium difengpi BN Chang (Schisandraceae) Stem Bark and its Constituent Compounds against Aedes aegypti L

Purpose: To determine the larvicidal activity of the essential oil derived from Illicium difengpi B.N. Chang stem bark (Schisandraceae) and its major constituents against the larvae of Aedes aegypti L.Methods: Essential oil of I. difengpi stem bark was obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromaotography-mas spectrometry (GC-MS). The activity of the essential oil and its major constituents was evaluated, using World Health Organization (WHO) procedures, against the fourth instar larvae of A. aegypti for 24 h, and larval mortalities recorded at essential oil/compound concentrations ranging from 6.0 - 200 μg/mL.Results: A total of 36 components of the essential oil of I. difengpi were identified. The principal compounds are safrole (18.21 %), linalool (13.47 %), 1,8-cineole (12.84 %), and myristicin (8.06 %) followed by α-terpineol (4.77 %), β-pinene (4.45 %) and 4-terpineol (4.38 %). The essential oil exhibited larvicidal activity against A. aegypti with LC50 (median lethal concentration) of 31.68 μg/mL. The major constituents, myristicin, safrole, and 1, 8-cineole, exhibited LC50 of 15.26, 39.45, and 72.18 μg/mL, respectively.Conclusion: The findings obtained indicate that the essential oil of I. difengpi and its major constituents have potentials for use in the control of A. aegypti larvae and may therefore be useful in the search for newer, safer and more effective natural compounds as larvicides.Keywords: Illicium difengpi, Aedes aegypti, Larvicidal activity, Myristicin, Safrole, 1,8-Cineole, Linaloo

Promoting positive development of migrant children in Hangzhou : pioneering experience and preliminary evaluation findings

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Major and trace-element zoning in metamorphic garnets and their metamorphic process implications

This article introduces some of the modern techniques for analyzing compositional zonings/profiles in metamorphic garnet, emphasizing the significance of two-dimensional composition X-ray mapping for such investigations. The compositional zonings/profiles in garnet can be divided into uniform-changed type and abrupt-changed type. The uniform-changed type is formed in equilibrium-controlled growth; the abrupt-changed type may be subdivided into step-changed subtype and spike-changed subtype, the forming mechanism of each is relatively complex. The step-changed subtype may be related to the processes such as poly-metamorphism, p-T changes with large magnitudes, and changes in metamorphic reactions or disturbance of crustal melting. As an example, the petrography, the major-and trace-element zoning and distribution patterns of REEs in the garnet from the Xiaomiao Group in the East Kunlun Mountains suggest two episodes of regional metamorphism.published_or_final_versio

Desensitization of T lymphocyte function by CXCR3 ligands in human hepatocellular carcinoma

Aim: Despite the presence of lymphocyte infiltration, human hepatocellular carcinoma (HCC) is typically a rapidly progressive disease. The mechanism of regulation of lymphocyte migration is poorly understood. In this study, we investigated various factors regulating T cell migration in HCC patients. We examined serum CXC chemokine levels in HCC patients and demonstrated the production of CXC chemokines by HCC cell lines. We determined the effect of both HCC patient serum and tumor cell conditioned supernatant upon lymphocyte expression of chemokine receptor CXCR3 as well as lymphocyte migration. Lastly, we examined the chemotactic responses of lymphocytes derived from HCC patients. Methods: The serum chemokines IP-10 (CXCL10) and Mig (CXCL9) levels were measured by cytometric bead array (CBA) and the tumor tissue IP-10 concentration was measured by ELISA. The surface expression of CXCR3 on lymphocytes was determined by flow cytometry. The migratory function of lymphocytes to the corresponding chemokines was assessed using an in vitro chemotactic assay. Phosphorylation of extracellular signal-regulated kinase (ERK) was determined by Western blot analysis. Results: Increased levels of IP-10 and Mig were detected in HCC patient serum and culture supernatants of HCC cell lines. The IP-10 concentration in the tumor was significantly higher than that in the non-involved adjacent liver tissues. HCC cell lines secreted functional chemokines that induced a CXCR3-specific chemotactic response of lymphocytes. Furthermore, tumor-cell-derived chemokines induced initial rapid phosphorylation of lymphocyte ERK followed by later inhibition of ERK phosphorylation. The culture of normal lymphocytes with HCC cell line supernatants or medium containing serum from HCC patients resulted in a significant reduction in the proportion of lymphocytes exhibiting surface expression of CXCR3. The reduction in T cell expression of CXCR3 resulted in reduced migration toward the ligand IP-10, and both CD4 + and CD8 + T cells from HCC patients exhibited diminished chemotactic responses to IP-10 in vitro compared to T cells from healthy control subjects. Conclusion: This study demonstrates functional desensitization of the chemokine receptor CXCR3 in lymphocytes from HCC patients by CXCR3 ligands secreted by tumor cells. This may cause lymphocyte dysfunction and subsequently impaired immune defense against the tumor. © 2005 The WJG Press and Elsevier Inc. All rights reserved.published_or_final_versio

Comparison of evolutionary rates in the mitochondrial DNA cytochrome b gene and control region and their implications for phylogeny of the Cobitoidea (Teleostei : Cypriniformes)

It is widely accepted that mitochondrial DNA (mtDNA) control region evolves faster than protein encoding genes with few exceptions. In the present study, we sequenced the mitochondrial cytochrome b gene (cyt b) and control region (CR) and compared their rates in 93 specimens representing 67 species of loaches and some related taxa in the Cobitoidea (Order Cypriniformes). The results showed that sequence divergences of the CR were broadly higher than those of the cyt b (about 1.83 times). However, in considering only closely related species, CR sequence evolution was slower than that of cyt b gene (ratio of CR/cyt b is 0.78), a pattern that is found to be very common in Cypriniformes. Combined data of the cyt b and CR were used to estimate the phylogenetic relationship of the Cobitoidea by maximum parsimony, neighbor-joining, and Bayesian methods. With Cyprinus carpio and Danio rerio as outgroups, three analyses identified the same four lineages representing four subfamilies of loaches, with Botiinae on the basal-most clade. The phylogenctic relationship of the Cobitoidea was ((Catostomidae + Gyrinocheilidae) + (Botiinae + (Balitorinae + (Cobitinae + Nemacheilinae)))), which indicated that Sawada's Cobitidae (including Cobitinae and Botiinae) was not monophyletic. Our molecular phylogenetic analyses are in very close agreement with the phylogenetic results based on the morphological data proposed by Nalbant and Bianco, wherein these four subfamilies were elevated to the family level as Botiidae, Balitoridae, Cobitidae, and Nemacheilidae. (c) 2005 Elsevier Inc. All rights reserved.It is widely accepted that mitochondrial DNA (mtDNA) control region evolves faster than protein encoding genes with few exceptions. In the present study, we sequenced the mitochondrial cytochrome b gene (cyt b) and control region (CR) and compared their rates in 93 specimens representing 67 species of loaches and some related taxa in the Cobitoidea (Order Cypriniformes). The results showed that sequence divergences of the CR were broadly higher than those of the cyt b (about 1.83 times). However, in considering only closely related species, CR sequence evolution was slower than that of cyt b gene (ratio of CR/cyt b is 0.78), a pattern that is found to be very common in Cypriniformes. Combined data of the cyt b and CR were used to estimate the phylogenetic relationship of the Cobitoidea by maximum parsimony, neighbor-joining, and Bayesian methods. With Cyprinus carpio and Danio rerio as outgroups, three analyses identified the same four lineages representing four subfamilies of loaches, with Botiinae on the basal-most clade. The phylogenctic relationship of the Cobitoidea was ((Catostomidae + Gyrinocheilidae) + (Botiinae + (Balitorinae + (Cobitinae + Nemacheilinae)))), which indicated that Sawada's Cobitidae (including Cobitinae and Botiinae) was not monophyletic. Our molecular phylogenetic analyses are in very close agreement with the phylogenetic results based on the morphological data proposed by Nalbant and Bianco, wherein these four subfamilies were elevated to the family level as Botiidae, Balitoridae, Cobitidae, and Nemacheilidae. (c) 2005 Elsevier Inc. All rights reserved

Searching for sterile neutrinos in ice

Oscillation interpretation of the results from the LSND, MiniBooNE and some other experiments requires existence of sterile neutrino with mass $\sim 1$ eV and mixing with the active neutrinos $|U_{\mu 0}|^2 \sim (0.02 - 0.04)$. It has been realized some time ago that existence of such a neutrino affects significantly the fluxes of atmospheric neutrinos in the TeV range which can be tested by the IceCube Neutrino Observatory. In view of the first IceCube data release we have revisited the oscillations of high energy atmospheric neutrinos in the presence of one sterile neutrino. Properties of the oscillation probabilities are studied in details for various mixing schemes both analytically and numerically. The energy spectra and angular distributions of the $\nu_\mu-$events have been computed for the simplest $\nu_s-$mass, and $\nu_s - \nu_\mu$ mixing schemes and confronted with the IceCube data. An illustrative statistical analysis of the present data shows that in the $\nu_s-$mass mixing case the sterile neutrinos with parameters required by LSND/MiniBooNE can be excluded at about $3\sigma$ level. The $\nu_s- \nu_\mu$ mixing scheme, however, can not be ruled out with currently available IceCube data.Comment: 41 pages, 16 figures. Accepted for publication in JHEP. Minor changes from the previous versio

Identification of genes differentially expressed in Jining Grey and Liaoning Cashmere goats ovaries

To search for genes controlling high prolificacy of Chinese indigenous goats, differential display reverse transcription-polymerase chain reaction (DDRT-PCR) was used to screen differentially expressed cDNA bands in the sexually matured ovaries of 3-year-old prolific Jining Grey goats and monotocous Liaoning Cashmere goats with 24 combinations of three anchored primers and eight arbitrary primers. 22 expressed sequence tags (ESTs) were proved to be the positive bands by Northern hybridization. They comprised 10 known ESTs and 12 ESTs without homologous sequences in the GenBank. These results indicate that several genes such as GATA-4, metallothionein-like protein, CAT genes and unknown ESTs (CV983340 and CV983341) were expressed only in Jining Grey goats.Keywords: Differential display reverse transcription-polymerase chain reaction, goat, ovary, prolificacyAfrican Journal of Biotechnology Vol. 12(27), pp. 4408-441

EM23, a natural sesquiterpene lactone, targets thioredoxin reductase to activate JNK and cell death pathways in human cervical cancer cells

Sesquiterpene lactones (SLs) are the active constituents of a variety of medicinal plants and found to have potential anticancer activities. However, the intracellular molecular targets of SLs and the underlying molecular mechanisms have not been well elucidated. In this study, we observed that EM23, a natural SL, exhibited anti-cancer activity in human cervical cancer cell lines by inducing apoptosis as indicated by caspase 3 activation, XIAP downregulation and mitochondrial dysfunction. Mechanistic studies indicated that EM23-induced apoptosis was mediated by reactive oxygen species (ROS) and the knockdown of thioredoxin (Trx) or thioredoxin reductase (TrxR) resulted in a reduction in apoptosis. EM23 attenuated TrxR activity by alkylation of C-terminal redox-active site Sec498 of TrxR and inhibited the expression levels of Trx/TrxR to facilitate ROS accumulation. Furthermore, inhibition of Trx/TrxR system resulted in the dissociation of ASK1 from Trx and the downstream activation of JNK. Pretreatment with ASK1/JNK inhibitors partially rescued cells from EM23-induced apoptosis. Additionally, EM23 inhibited Akt/mTOR pathway and induced autophagy, which was observed to be proapoptotic and mediated by ROS. Together, these results reveal a potential molecular mechanism for the apoptotic induction observed with SL compound EM23, and emphasize its putative role as a therapeutic agent for human cervical cancer.published_or_final_versio

A fMRI Study of Correlation Between Acupoints and Brain Cortical Sites Involved in Language Functions

published_or_final_versio

Seroprevalence of Mycoplasma bovis infection in dairy cows in subtropical southern China

The seroprevalence of Mycoplasma bovis infection in dairy cows in Guangxi Zhuang Autonomous Region (GZAR) in subtropical southern China was surveyed between June 2009 and March 2010. A total of 455 serum samples of dairy cows were collected from 6 districts in 4 different cities, and examined for M. bovis antibodies with the indirect enzyme-linked immunosorbent assay (ELISA) using a commercially available kit. The overall seroprevalence of M. bovis infection in dairy cows was 7.69% (35/455). Three year-old dairy cows had the highest seroprevalence (15.0%), followed by dairy cows of 4 year-old (11.1%). Dairy cows with the history of 5 pregnancies had the highest seroprevalence (33.3%). However, no statistically significant association was found between M. bovis infection and age or number of pregnancies (p > 0.05). All the aborting dairy cows were negative for M. bovis antibodies, suggesting that bovine abortion may have no association with M. bovis infection in GZAR. These results indicate that M. bovis infection in dairy cows was widespread in GZAR, and integrated strategies and measures should be performed to control and prevent M. bovis infection and disease outbreak.Key words: Mycoplasma bovis, seroprevalence, dairy cows, Guangxi Zhuang Autonomous Region (GZAR), China, enzyme-linked immunosorbent assay (ELISA)